Ing was excised from the gel, digested and analyzed by mass

Ing was excised from the gel, digested and analyzed by mass spectrometry. By using the Mascot program, 22 proteins could be identified with a significant score (Table 3), including 16 upand 4 down-regulated, 1 new and 1 equally expressed in both genotypes (Figure 2). The other proteins were in insufficient amounts for the identification analysis or did not return reliable matches when using the Mascot program. This probably occurs due to a low protein quantity and/or low ionization capacity of molecular components present in the samples analyzed. It is also possible that, considering the high amount of "no hits" obtained in the genomic analysis, protein sequences matching the peptides searched were not available in public databases. The peptide sequences obtained were also analyzed using the Blastp program. Spots PL1 and PL2 (up-regulated in Prata Ligeiro) were identified as hypothetical proteins which contain Ricin Brelated lectin domain. Other up-regulated hypothetical proteins were also identified and include protein spots PL34, PL45 and PL51. Spot PL45 and PL51 were expressed 2.6 and 4.5 fold, respectively, in the tolerant genotype (Figure 2), indicating that these proteins may play an important role in drought tolerance. Spot PL57 wasPage 7 of(page number not for citation purposes)BMC Genomics 2008, 9:http://www.biomedcentral.com/1471-2164/9/Table 2: Genes detected exclusively in roots of the susceptible genotype (IRAT20) SSH libraryEncoded protein Proteins of known function T complex 11 family protein Protein kinase domain containing protein Protein disulphide isomerase family protein TPR-like domain containing protein Protein kinase Pinoresinol-lariciresinol reductase TH1 Smr protein; MutS2 c- terminal domain containing protein SIPL protein (Membrane-type 1 matrix metalloproteinase cytoplasmic tail binding protein-1) Similar to CG 9092- PA Putative ATP-dependent Clp protease ATP-binding subunit ClpX1 (CLPX) Cytocrome P450 family protein Preprotein translocase subunit sec Y, chloroplast precursor Vacuolar H+ pyrophosphatase Similar to UPF 0139 protein CGI-140 60 kDa inner membrane insertion protein family protein Glyceraldehyde-3-phosphate dehydrogenase (Fragment) Similar to splicing coativator subunit SRm 300 Cysteine synthase, mitocondrial precursor TPR-like domain containing protein HCO3-transporter Banched chain amino-acid aminotransferase-like protein 3 Beta tubulin (fragment) HAT dimerisation domain containing protein Urease accessory protein G Glycoside hydrolase, family 47 protein WRKY transcription factor 82 Tubby family protein Ribosomal protein L41 family protein Granule-bound PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/16989806 starch synthase I, chloroplast precursor Putative RNA polymerase I transcription factor RRN3 Aconitate hydratase, cytoplasmic (Citrate hydro-lyase) (Aconitase) Short chain alcohol dehydrogenase-like Putative ubiquitin-conjugating enzyme E2 Peptidase s26A signal peptidase I family protein Protein of unknown function Unknown protein Hypothetical protein Hypothetical protein Hypothetical protein Hypothetical protein Hypothetical protein Hypothetical protein Hypothetical protein Hypothetical protein Hypothetical protein Hypothetical protein Hypothetical protein Hypothetical protein Capecitabine Hypothetical protein Hypothetical protein Hypothetical protein Hypothetical protein Hypothetical protein Hypothetical protein Hypothetical protein Hypothetical protein Hypothetical protein Hypothetical proteinHomologous organismAccession numberOryza sativa Oryza sativa Oryz.