A actin, isoform alpha-enolase of alpha-enolase (ENO1), phosphoglycera…

A actin, isoform alpha-enolase of alpha-enolase (ENO1), phosphoglycerate mutase 2 (PGAM2), isoform M1 of pyruvate kinase isozymes M1/M2(PKM2) and isoform 8 of Titin. Neither cTnI nor cTnT were detected possibly because of the lower level of cTnI and cTnT release with mild ischemic injury of myocardium, their late release, or few detectable peptides for them after use of tryptic digestion. Four proteins expressed abundantly in heart were identified including Cysteinerich protein 2,S100 calcium binding protein A1, 14 kDa phosphohistidine phosphatase and mesenchymal stem cell protein DSC92. In additional, those identified proteins including Glucose-6-phosphate isomerase (GPI), Creatine kinase M-type (CKM), Malate dehydrogenase (MDH1), Creatine kinase B-type (CKB), (heart-type) D(+)-Galactosamine (hydrochloride) Fatty acid-binding protein (FABP3), Glycerol-3-phosphate dehydrogenase [NAD+](GPD1), PGAM2, MB, Superoxide dismutase(SOD1), Adenylate kinase 1(AK1), Isoform 1 of Triosephosphate isomerase(TPI1), Phosphatidylethanolamine-binding protein 1(PEBP1), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Aspartate aminotransferase,(GOT1) and Four and a half LIM domains 1 variant(FHL1) were also detected in coronary sinus plasma of planned myocardial infarction patients by Addona PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/25751659 et al [18].Table 2 Part of the identified proteins that may be interesting in potential biomarker discovery of early ischemia in myocardiumProtein name Isoform M1 of Pyruvate kinase isozymes M1/M2 Actin, alpha cardiac muscle 1 Aspartate aminotransferase 1 Creatine kinase M-type Heart -fatty acid-binding protein, Glyceraldehyde-3-phosphate dehydrogenase L-lactate dehydrogenase B chain Myoglobin Isoform 1 of Triosephosphate isomerase Isoform 8 of Titin Glucose-6-phosphate isomerase Isoform 1 of Gelsolin Phosphoglycerate mutase 2 Adipocyte -fatty acid-binding protein Adenylate kinase 1 Creatine kinase B-type Four and a half LIM domains 1 variant Malate dehydrogenase, cytoplasmic Phosphatidylethanolamine-binding protein 1 PGM1 65 kDa protein Superoxide dismutase Function metabolism cytoskeleton metabolism (amino acid) energ transduction lipid transport metabolism (glycolysis) metabolism (glycolysis) oxygen transport metabolism (glycolysis) cytoskeletal activity metabolism (glycolysis) cytoskeletal activity metabolism (glycolysis) lipid transport ATP regeneration kinase activity protein binding metabolism (glycolysis) protease inhibitor magnesium ion binding oxidoreductase Distinct peptide 12 8 6 15 9 14 14 17 12 4 5 9 2 9 5 2 5 12 8 3 1 MW(Da) 58,062.4 42,019.2 46,247.7 43,101.4 14,858.1 36,053.4 36,638.7 17,183.9 30,791.2 3829,893.5 63147.5 85697.9 28766.3 14719 23410.9 42644.5 33578.9 36426.3 21056.9 64560.9 15935.8 yes yes yes yes yes yes Organ specific yes yes yes yes yes yes yes Clinical marker yesLi et al. Proteome Science 2012, 10:21 http://www.proteomesci.com/content/10/1/Page 6 ofFigure 4 The relationship between the mean of peak intensities (MPI) and ischemic time. A. Presented was the relationship between the mean of peak intensities and ischemic time in hemoglobin. B. Presented was the relationship between the mean of peak intensities and ischemic time in L-lactate dehydrogenase B.The relative protein levels were compared by the Spectrum Mill software according to MPI of the component peptides. Trends in MPI of proteins matched by database searches can be used to determine which proteins were released from the tissue. For examples, the MPI of the plasma proteins, such as hemoglobin, followed a.