Increased in ENO1Table 1 Upregulation of ENO1 protein in NSCLC specime…

Increased in ENO1Table 1 Upregulation of ENO1 protein in NSCLC specimens compared to non-cancerous lung specimensGroup Cases (n) Protein expression (n) Positive expression NSCLC NormalChi-square test.P valueNegative expression 21 12 0.5534To examine the effect of ENO1 on cell migration and invasion, a transwell apparatus and Boyden chamber coated with Matrigel were used. After 10-h incubation, an elevated number of migrated cells were observed in A549-ENO1 compared to its control cells and untreated cells (P PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/6833145 control. Bars show the mean ?SD; (*P Capivasertib NSCLCTo further study the mechanism by which ENO1 regulates cell proliferation, migration, and invasion, the protein levels of cell cycle and EMT-associated genes wereexamined in A549 and SPCA-1 cells with stably overexpressed or suppressed ENO1. In ENO1 stably overexpressing A549 cells, activation of p-Rb (ser 780) was increased as well as the elevated expression of cyclin D1, cyclin E1, and c-Myc. In contrast, the expression of p21 was inhibited. Stably knocking down endogenous ENO1 expression in SPCA-1 inhibited the activation of p-Rb (ser 780), and the expression of cyclin D1, cyclin E1, and c-Myc were decreased, while levels of p21 were upregulated (Figure 5A). We also found that upregulated ENO1 expression elevated the expression of EMT marker genes including snail, vimentin, and N-cadherin, yet inhibited E-cadherin in A549 cells. Conversely, downregulated ENO1 expression in SPCA-1 cells inhibited the expression of.